Special Feature

Protein databases from India

[Nature India Special Issue: Proteomics Research in India]

Harsha Gowda1, 2, Aditi Chatterjee1, 2 & T. S. Keshava Prasad1, 2, 3

doi:10.1038/nindia.2015.118 Published online 27 August 2015

In recent years, there has been an exponential surge in biological data. The advent of high-resolution instruments with better sensitivities and new-age software suites have made it easier for data analysts and researchers to use omics approaches in biomedical investigations. Using such approaches, researchers are generating vast amounts of molecular data that provide a platform for several novel hypotheses.

Biological databases play an important role in assimilating large amounts of data and enabling users to access this information using dynamic query systems. These diverse datasets also serve as a medium to identify enzyme-substrate- and metabolic- networks involved in various biological processes, which can be helpful in molecular diagnostics and therapeutics for human diseases; and genomic selection of better biological traits in crop plants and dairy animals. Databases also provide a scaffold for the execution of regular updates, international data exchange and global meta-analysis.

Databases of protein features, resources for signaling and metabolic pathways that drive specific biological processes, and repositories of disease-specific molecular level alterations will help researchers apply systems biology approaches to unearth mechanisms or leads with greater biological significance.

Large protein databases

Leading the Indian proteomic database revolution is Bangalore-based Institute of Bioinformatics (IOB; http://ibioinformatics.org) with its flagship Human Protein Reference Database (HPRD) and other noteworthy platforms – the Human Proteinpedia, Human Proteome Map, a database on molecular alterations reported in pancreatic cancers and several human signaling pathways.

The HPRD (http://www.hprd.org/) has highly curated information on a non-redundant set of 30,047 human proteins, which include 40, 042 protein-protein interactions and 1, 09, 518 post-translational modifications1. A number of biomedical scientists use HPRD either directly by downloading data or indirectly by using the information available in RefSeq and Entrez Gene databases of the National Center for Biotechnology Information (NCBI) and University of California Santa Cruz (UCSC) genome browser.

The Human Proteinpedia (http://www.humanproteinpedia.org) provided a platform for over 200 proteomic laboratories for storing, sharing and dissemination of multidimensional proteomic datasets, even before publication2, 3. Data from Human Proteinpedia is also made available to the larger scientific community through HPRD.

Human Proteome Map (http://humanproteomemap.org/) is another interactive web portal, which represents the largest mass spectrometry- derived label-free quantitative proteomic data from 30 different human tissues4. The Plasma Proteome Database (PPD, http://plasmaproteomedatabase.org/), initiated as a part of the Human Plasma Proteome Project of Human Proteome Organization, contains information on 10, 546 proteins detected in human serum/ plasma5.

The institute has also created a highly curated database on molecular alterations reported in pancreatic cancers, initially published as a compendium of overexpressed proteins, and followed up with the database of molecular alteration at mRNA, protein and miRNA6.

IOB’s centralised resource for human signaling pathways is called NetPath (http://www.netpath.org). NetPath contains manually curated data for 36 signaling pathways including prolactin7, gastrin8, corticotropin-releasing hormone (CRH)9, fibroblast growth factor-1 (FGF1)10, interleukins (IL-1, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, and IL-11), brain-derived neurotrophic factor (BDNF)11, leptin12, oncostatin-M13 and RANKL14, among others. IOB scientists have curated several signaling pathways such as Delta-Notch, EGFR1, Hedgehog, TNF-alpha and Wnt for Cancer Cell Map (http://cancer.cellmap.org/cellmap/), which is a database of human cancer focused pathways developed by Memorial Sloan-Kettering Cancer Center in New York, USA.

A slimmer version of signaling pathways annotated in NetPath were gathered to form NetSlim (http://www.netpath.org/netslim/), which comprises a graphical network of core signaling reactions15.

The Bioinformatics Centre at Institute of Microbial Technology in Chandigarh has created many web servers and protein databases to perform structure and function of proteins based on their amino acid sequences, potential MHC class I and II binding regions in antigens, subcellular localisation and classification of eukaryotic and prokaryotic proteins, identification of bacterial toxins and several analytical tools for pattern finding in genome annotation. The IMT group has created a curated database of proteins associated with cervix cancer – CCDB16; a database of anticancer peptides and proteins called CancerPPD17, and a database of hemolytic and non-hemolytic peptides — Hemolytik.

The National Centre of Biological Sciences (NCBS) in Bangalore has created a number of publicly available databases of protein family trees by analysis of protein motifs and domains. These resources include SMOS.2, LenVarDB, 3PFDB, SUPFAM; GenDiS18, MegaMotifbase, iMOTdb and PASS219. A database on disulphide bonds (DSDBASE)20, A database of olfactory receptors (DOR)21 and a resource for transcription factors responding to stress in Arabidopsis thaliana (STIFDB)22 were also developed by the team.

NCBS has also developed the Database of Quantitative Cellular Signaling (DOQCS), which represents the collection of basic models of signaling pathways23, 24, 25, 26.

Researchers at the Indian Institute of Science have created a number of protein databases related to structure and function of protein kinases. These include ‘KinG’, a database of kinases27, NrichD28, PALI, PRODOC and MulPSSM29, resources of protein domains and alignments.

Databases for analysis of secondary structures of proteins, which include Conformation Angles DataBase of proteins (CADB), a web‐based database of Transmembrane Helices in Genome Sequences (THGS)30 and Secondary Structural Elements of Proteins (SSEP)31 have also been received well by researchers worldwide.

At the Center of Bioinformatics in Pondicherry University, researchers have developed a number of protein databases including Peptide Binding Protein Database, Immune Epitope Prediction Database & Tools, Structural Epitope Database (SEDB)32, Clostridium-DT(DB)33, a comprehensive database for potential drug targets of Clostridium difficile and Viral Protein Structural Database (VPDB)34.

A manually curated database of rice proteins (http://www.genomeindia.org/biocuration) was developed by a team from the University of Delhi South Campus and is an important plant protein database from India35.

Future outlook

India is uniquely positioned to take a lead in developing and maintaining world class biological databases. A large base of human resource in biological sciences as well as software technology serves as an advantage. Although there are independent efforts from different research labs in India to build and maintain biological databases, there hasn’t been a dedicated effort to do it at a scale that is required to build and maintain world class databases much like how NCBI and EBI are doing for several years.

There are several companies in India that are offering annotation and database services to industries, which clearly demonstrates existence of such capability in India. Much of the data that is being generated within India is also not organised for the immediate use by fellow researchers.

Dedicated funding from government and corporates for academic research groups with demonstrated capability in developing and maintaining world class databases could be a good starting point. Such resources will become a necessity in the future as the amount of data being generated continues to grow.

1Institute of Bioinformatics, International Technology Park, Bangalore, India (harsha@ibioinformatics.org, aditi@ibioinformatics.org, keshav@ibioinformatics.org). 2YU-IOB Center for Systems Biology and Molecular Medicine, Yenepoya University, Mangalore, India. 3NIMHANS-IOB Proteomics and Bioinformatics Laboratory, Neurobiology Research Centre, National Institute of Mental Health and Neurosciences, Bangalore, India.


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